Table 2.

RNA and DNA-binding results

	RNA aptamer			tetO dsDNA
	SEC	EMSA	ITC	SEC	ITC
Construct	Complex formation	K d [nM]	K d [nM]	Complex formation	K d [nM]
TetR	Yes	80	5.6	yes	51.1
TetR–R28A	No	n.b.a	n. b.	no	n. b.
TetR–Q38A	Yes	>500b	26.2	yes	432.7
TetR–Y42A	Yes	n.b.	13.9 × 103	no	n.b.
TetR–R28AY42A	No	n.b.	–c	no	–

^aNo binding detectable.

^bAccurate determination of K_d not possible with the reported experimental setup.

^cNot determined.