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. 2020 Feb 4;48(6):3071–3088. doi: 10.1093/nar/gkaa055

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© The Author(s) 2020. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 2. — A GLN4 tRNA synthetase shut-off induces a reporter of GCN4 translation. The effect of GLN4 tRNA synthetase shut-off, using doxycycline, on uncharged tRNA accumulation and thus GCN4 activation was explored using the GCN4-lacZ reporter plasmids. The effect of doxycycline on GCN4 induction was tested in a wild-type cell (BY4742, black bars, left-most in each block), and in a tetO-GLN4 strain (remaining bars). Error bars represent ±1 standard deviation, n = 3. Significance at the P < 0.05 level is indicated by an asterisk. Plasmid p180 measures a GCN4 response, relative to the negative (p226) and positive (p227) controls.