Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Jan 31;48(6):3328–3342. doi: 10.1093/nar/gkaa062

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2020. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Figure 1. — Three classes of the overall structures of the FANCA–FANCG complex. (A) Cryo-EM map of the FANCA CTD dimer at an average resolution of 3.35 Å. Each FANCA molecule is shown in orange (FANCA) and green (FANCA’). The arc-shaped CTD is divided into the N-terminal half (arc^N) and the C-terminal half (arc^C). (B) Cryo-EM map (gray) of the FANCA CTD dimer complexed with a FANCG (purple) molecule at 4.6 Å. FANCG CTD packs at the C-terminal end of FANCA. Orientation of the CTD dimer is same as that in (A). (C) Cryo-EM map (gray) of the FANCA and FANCG complex at 4.84 Å. FANCG’ is bound at the FANCA’ NTD (yellow). (D) Overall scheme of the subdomain composition in FANCA and FANCA’. Each subdomain is painted with same colors as in (A) to (E). (E) Two views of the 3.46 Å structure of the FANCA CTD dimer. Left, A 2-fold rotation axis is along the horizontal axis. Each HEAT repeat consists of the HA and HB helices that forms inner and outer surfaces, respectively. The central axes (along each H8B helix) of two solenoids are arranged in ∼80°. Right, View orthogonal to the left view looking down the 2-fold axis which is along the H8B helices from the two FANCA molecules. The CTD structure is divided into the N-terminal helical subdomain (H1 to linker helix), arc^N (H3 to HM1) and arc^C (H7 to H14) subdomains. The helical subdomain of FANCA’ is shown in cyan. The arc^N and arc^C subdomains of FANCA CTD are colored in light orange and orange, respectively. The arc^N and arc^C subdomains of FANCA’ CTD are shown in light green and green, respectively.