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. 2020 Feb 21;48(6):3134–3155. doi: 10.1093/nar/gkaa100

Table 2.

Free energy parameters for G/U pairs

5′U-3′G 5′G-3′U
(akin to Case 1) (akin to Case 2)
5′G-C3′ −2.5 −1.5
5′C-G3′ −2.1 −1.4
5′A-U3′ −1.4 −0.6
5′U-A3′ −1.3 −1.0

General contributions of G/U pairs to overall helix free energy (ΔG°37 kcal/mol) with respect to adjacent WC base pairs indicated to the left. Values applicable to Case 1 and Case 2 contexts are indicated.