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Effects of key residue mutation on DNA polymerization activity of NrS-1 polymerase. (A) In vitro DNA polymerization assays catalyzed by mutant proteins of NrS-1 polymerase. (B) Comparison of P1 and P2 products generated by WT and mutant proteins of NrS-1 polymerase. (C) In vitro DNA polymerization assays catalyzed by WT and K453A mutant of NrS-1 polymerase.
