Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Jan 25;48(6):3029–3041. doi: 10.1093/nar/gkaa043

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2020. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

PMC Copyright notice

Figure 3. — Telomeres detach from NE in bqt4Δ quiescent cells. Visualization of telomeric foci, spindle pole body (SPB) and the nuclear envelope (NE) by live microscopy in vegetative (Vg) and quiescent (G0) cells for the WT and bqt4Δ strains. (A) Taz1-GFP (green), Cdc11-CFP (red) and Amo1-GFP (green) are used to visualize the telomeres, the SPBs and the NE, respectively. Green arrows and red arrows indicate telomeres and SPB, respectively. (B) Percentage of cells that display one or several telomeric foci. (C) Percentage of WT and bqt4Δ cells that display a unique telomeric focus with time in quiescence. (D) Taz1-GFP hypercluster localization relative to the NE (Amo1-GFP). Z-stacks images of living cells and scoring of the position of the Taz1-GFP hypercluster in one of the three equal concentric zones of the nucleus with respect to Amo1-GFP in the focal plane of the GFP focus. (E) Distance to NE of the telomeric foci in Vg and G0 cells. P-values are indicated. This experiment was repeated in triplicate and for each experiment >100 nuclei with a hyper-cluster were analyzed (***P < 0.0005). Error bars represent SEM.