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. 2020 Feb 3;48(6):e31. doi: 10.1093/nar/gkaa030

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© The Author(s) 2020. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 3. — Mappings of genomic interactions between replicated ChIA-PET experiments are predominantly unambiguous. The great majority of interactions in replicate 1 that overlapped with interactions in replicate 2 only overlapped with one interaction, leading to an unambiguous assignment of corresponding replicated interactions (green bars). Unsurprisingly, the number of ambiguous mappings (interactions in replicate 1 that overlap with more than one interaction in replicate 2) increases when the maximum acceptable gap is increased, the tolerated distance between anchors to still be considered overlapping.