Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Feb 11;48(6):2969–2981. doi: 10.1093/nar/gkaa073

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Published by Oxford University Press on behalf of Nucleic Acids Research 2020.

This work is written by (a) US Government employee(s) and is in the public domain in the US.

PMC Copyright notice

Figure 5. — PRC2 binding depends on PRC2 complex composition. (A) Cartoons illustrating the measured PRC2 moieties. (top) The ‘5mer’ complex consists of EZH2 (red), EED (blue), SUZ12 (yellow), AEBP2 (purple) and RBBP4 (green). (middle) The catalytic moiety of PRC2 consists of EZH2, the VEFS domain of SUZ12, and EED with the 81 N-terminal amino acids deleted. (bottom) The regulatory moiety of PRC2 consists of SUZ12ΔVEFS, AEBP2, and RBBP4. (B) Representative 2 × 2 μm² images at 30 and 90 nM concentrations of the regulatory moiety of PRC2. (C) Probability of observing a PRC2-DNA complex (P_bound) as a function of PRC2 concentration for the regulatory (purple), catalytic (gray) and 5-mer (green) moieties of PRC2. Concentrations below the were used to enhance the signal. The triple asterisk represents a statistical significance of P < 0.001. The average N_molecules at 0, 30 and 90 nM were greater than 80, 90 and 200, respectively, and the error bars represent the standard error in the mean. Gray shading indicates the false positive rate, based on the no added PRC2 control.

Inline graphic — PRC2 binding depends on PRC2 complex composition. (A) Cartoons illustrating the measured PRC2 moieties. (top) The ‘5mer’ complex consists of EZH2 (red), EED (blue), SUZ12 (yellow), AEBP2 (purple) and RBBP4 (green). (middle) The catalytic moiety of PRC2 consists of EZH2, the VEFS domain of SUZ12, and EED with the 81 N-terminal amino acids deleted. (bottom) The regulatory moiety of PRC2 consists of SUZ12ΔVEFS, AEBP2, and RBBP4. (B) Representative 2 × 2 μm² images at 30 and 90 nM concentrations of the regulatory moiety of PRC2. (C) Probability of observing a PRC2-DNA complex (P_bound) as a function of PRC2 concentration for the regulatory (purple), catalytic (gray) and 5-mer (green) moieties of PRC2. Concentrations below the were used to enhance the signal. The triple asterisk represents a statistical significance of P < 0.001. The average N_molecules at 0, 30 and 90 nM were greater than 80, 90 and 200, respectively, and the error bars represent the standard error in the mean. Gray shading indicates the false positive rate, based on the no added PRC2 control.