Fig. 2.

Bone marrow and blood leukocyte characterization by mass cytometry. The SPADE tree shows the global analysis of leukocyte populations in blood and bone marrow from uninfected and chronically SIV-infected macaques. a The topology of the SPADE tree is shown with the cluster number associated with each node and color according to manually annotated leukocyte populations. cDC, classical dendritic cells; pDC, plasmacytoid dendritic cells. b The tree was built using all samples and split according to the compartment and SIV status. Each dot represents a cluster with the size proportional to the cell abundance among all cells. SIV infection led to changes in the abundance of neutrophil clusters in both blood and bone marrow.