Table 1.
Inflammatory cell counts in bronchoalveolar lavage (BAL) fluid of C57BL/6 mice exposed to 162 μg of CBNPs at eight different post-exposure time-points. The number of cells is expressed as ± standard error of the mean (SEM) × 103.
| Time-points | Cell types | Number of cells × 103 |
|
|---|---|---|---|
| Control | CBNPs | ||
| 3 h | Neutrophils | 3.9± 1.2 | 26.7 ± 5.5⁎⁎ |
| Macrophages | 38.2 ± 8.3 | 46.7 ± 3.1 | |
| Eosinophils | 0.7 ± 0.2 | 0.6 ± 0.2 | |
| Lymphocytes | 0.4 ± 0.2 | 0.1 ± 0.1 | |
| Epithelial | 16.6 ± 2.7 | 42.6 ± 3.6⁎⁎ | |
| Total BAL cells | 59.7 ± 11.4 | 116.8 ± 7.7⁎⁎ | |
| 1 d | Neutrophils | 4.1 ± 1.0 | 123.4 ± 8.4⁎⁎⁎ |
| Macrophages | 39.8 ± 5.4 | 44.2 ± 8.4 | |
| Eosinophils | 1.9 ± 1.2 | 7.1 ± 2.4 | |
| Lymphocytes | 0.6 ± 0.2 | 1.0 ± 0.6 | |
| Epithelial | 11.0 ± 3.1 | 14.2 ± 2.2 | |
| Total BAL cells | 57.3 ± 3.9 | 189.8 ± 12.1⁎⁎⁎ | |
| 2 d | Neutrophils | 4.0 ± 2.3 | 97.4 ± 6.4⁎⁎⁎ |
| Macrophages | 56.8 ± 8.3 | 50.8 ± 5.0 | |
| Eosinophils | 2.3 ± 0.9 | 2.5 ± 1.0 | |
| Lymphocytes | 1.3 ± 0.4 | 1.6 ± 0.4 | |
| Epithelial | 22.4 ± 4.3 | 16.0 ± 1.9 | |
| Total BAL cells | 86.9 ± 11.4 | 168.4 ± 9.8⁎⁎ | |
| 3 d | Neutrophils | 2.0 ± 0.8 | 84.5 ± 6.0⁎⁎⁎ |
| Macrophages | 48.9 ± 13.5 | 65.0 ± 5.3 | |
| Eosinophils | 1.3 ± 0.4 | 3.7 ± 1.9 | |
| Lymphocytes | 0.6 ± 0.2 | 3.3 ± 1.1 | |
| Epithelial | 13.0 ± 3.3 | 14.5 ± 2.7 | |
| Total BAL cells | 65.9 ± 17.5 | 171.1 ± 7.1⁎⁎⁎ | |
| 4 d | Neutrophils | 0.4 ± 0.2 | 80.0 ± 9.0⁎⁎⁎ |
| Macrophages | 38.6 ± 8.2 | 62.5 ± 7.2⁎ | |
| Eosinophils | 0.8 ± 0.7 | 11.0 ± 2.9⁎ | |
| Lymphocytes | 0.2 ± 0.1 | 8.1 ± 1.4⁎⁎⁎ | |
| Epithelial | 9.3 ± 1.0 | 15.1 ± 4.3 | |
| Total BAL cells | 49.2 ± 9.3 | 176.8 ± 13.1⁎⁎⁎ | |
| 5 d | Neutrophils | 0.2 ± 0.1 | 90.6 ± 12.9⁎⁎⁎ |
| Macrophages | 40.0 ± 6.0 | 89.6 ± 6.5⁎⁎⁎ | |
| Eosinophils | 9.1 ± 5.6 | 20.4 ± 9.4 | |
| Lymphocytes | 1.5 ± 0.7 | 16.9 ± 6.6⁎ | |
| Epithelial | 17.4 ± 1.7 | 16.8 ± 4.7 | |
| Total BAL cells | 68.2 ± 10.6 | 234.3 ± 18.5⁎⁎⁎ | |
| 14 d | Neutrophils | 1.1 ± 0.4 | 29.2 ± 5.4⁎⁎⁎ |
| Macrophages | 53.4 ± 11.6 | 59.1 ± 9.5 | |
| Eosinophils | 0.4 ± 0.3 | 0.9 ± 0.3 | |
| Lymphocytes | 0.2 ± 0.1 | 1.6 ± 0.4⁎ | |
| Epithelial | 27.3 ± 6.7 | 26.3 ± 1.7 | |
| Total BAL cells | 98.8 ± 8.2 | 117.1 ± 15.4 | |
| 42 d | Neutrophils | 0.7 ± 0.3 | 30.5 ± 4.9⁎⁎⁎ |
| Macrophages | 37.5 ± 2.5 | 97.1 ± 10.8⁎⁎⁎ | |
| Eosinophils | 0.5 ± 0.2 | 3.8 ± 1.3 | |
| Lymphocytes | 0.0 ± 0.0 | 0.8 ± 0.7 | |
| Epithelial | 17.4 ± 2.2 | 30.3 ± 4.7 | |
| Total BAL cells | 56.1 ± 4.5 | 162.5 ± 19.7⁎⁎⁎ | |
h — hour; d — day.
⁎
p < 0.05.
⁎⁎
p < 0.01.
⁎⁎⁎
p < 0.001.