Fig. 4.

Expression of pluripotency factors in biPSCs. (A) Real-time PCR analysis of the relative RNA expression levels of pluripotency genes (Oct4, Sox2, cMyc, and Klf4) in biPSCs versus that in BEFs. Data were normalized to β-actin mRNA expression levels and error bars were calculated by standard deviation (SD), n = 3. (B) The immunofluorescence staining of pluripotency markers Oct4, Sox2, Nanog, TBX3, and TRA-1-60 of biPSC colonies are shown (fluorescence, upper; 4,6-diamidino-2-phenylindole, middle; bright field, bottom). (C) Western blot analysis of Oct4 and Sox2 proteins. BiPSCs protein expression levels of BEFs and mouse ESCs were used as negative and positive controls, respectively. β-Actin was used as the loading control. biPSC, bat-induced pluripotent stem cell; PCR, polymerase chain reaction; BEF, bat embryonic fibroblast; ESC, embryonic stem cell.