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. Author manuscript; available in PMC: 2020 Mar 29.
Published in final edited form as: J Vis Exp. 2019 Jan 17;(143):10.3791/59051. doi: 10.3791/59051

Figure 1: Morphology assessment of organoids in 3D culture.

Figure 1:

(A) Image collection and compression workflow for human primary prostate organoids acquired by a transmitted light inverted microscope with a motorized X/Y scanning stage and companion software. (B) Representative images of a single z-stack (left) vs. an EDF image (right) of a whole-well sample of human primary prostate organoids, showing that more organoids are in focus when multiple z-stacks are combined into a single projected image (scale bar = 1000 μm). (C) Representative images for area, circularity, and max/min ratio of morphologically dissimilar human primary prostate organoids which have the same area (scale bar = 200 μm).