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. 2017 Nov 10;29(48):484003. doi: 10.1088/1361-648X/aa90d0

Figure 2.

Figure 2.

CLPs have incomplete capsid shells independent of cargo length. (A) The amount of CLPs present during assembly and disassembly at increasing NaCl concentrations based on light scattering intensity. (B) Images of CLPs used for cryo image reconstruction. The arrows indicate non-spherical particles. Inset, rotationally and translationally averaged particle images showing different concentric densities between the 27mer CLP and the 48hp/48lin CLPs. (C) 2D images and class-averaging of 27mer, 48hp, and 48lin CLPs shows regions of discontinuity in the CP shell. (D) cryo-EM reconstructions imposing icosahedral symmetry show that 27mer CLPs have different surface arrangement of CP compared to CLPs assembled with 48hp and 48lin oligos.