Table 4. OPLS-DA coefficients derived from the NMR data of plasma metabolites obtained from (A) control, (B) 50% replacement group (50% of the uncontaminated cottonseed replaced with cottonseed contaminated with mycotoxins) and (C) 100% replacement group (100% of the uncontaminated cottonseed replaced with cottonseed contaminated with mycotoxins).
| Metabolitesb | ra | ||
|---|---|---|---|
| A vs. B (df = 7) | A vs. C (df = 7) | B vs. C (df = 7) | |
| Ala(Alanine): 1.48(d) | – | −0.669 | 0.763 |
| Lys(Lysine): 1.49(m), 1.74(m), 1.91(m) | 0.743 | – | 0.697 |
| Gln(Glutamine): 2.12(m), 2.42(m) | – | – | 0.733 |
| OAG(O-acetyl-glycoprotein): 2.13(s) | – | – | 0.668 |
| Cit(Citrate): 2.54(d), 2.68(d) | – | −0.748 | 0.695 |
| Cr(Creatine): 3.03(s),3.93(s) | – | – | 0.674 |
| Gly(Glycine): 3.56(s) | – | – | 0.731 |
| Glucose: 3.42(t), 3.54(dd), 3.71(t), 3.73(m), 3.84(m), 3.25(dd), 3.41(t), 3.46(m), 3.49(t), 3.90(dd), 4.65(d), 5.23(d) | – | – | 0.768 |
Notes.
a
Correlation coefficients where positive and negative signs indicate positive and negative correlations between the concentrations, respectively. Correlation coefficients of |r| > 0.666, 0.707, or 0.755 were used as the cutoff values for significant differences based on the discrimination significance at the P = 0.05 level, where df (degrees of freedom) = 7, 6, or 5; “-” indicates that the correlation coefficient |r| was less than 0.666, 0.707, or 0.755.
b
Multiplicity: s, singlet; d, doublet; t, triplet; q, quartet; dd, doublet of doublets; m, multiplet; br, broad resonance.