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. 2011 Dec 3;36(4):657–664. doi: 10.1016/j.dci.2011.11.008

Fig. 5.

Fig. 5

Type I IFNs are upregulated in P. alecto kidney cells upon poly I:C challenge. PaKiT03 cells were challenged for 3 h with the indicated concentrations of poly I:C, either by direct addition to the supernatant (treatment), or in combination with Lipofectamine 2000 (transfection). Levels of IFN-α, IFN-β and IFNλ (IL-28) mRNA were measured by qRT-PCR using the standard curve method. Results are given as fold-change relative to mock treatment or mock transfection, respectively. N = 4 replicate cultures. Error bars represent standard deviation.