Table 1.

RACE, full-length amplification, and qPCR primers used in this study.

Gene	Primer	Nucleotide sequence (5′–3′)	Application
TLR1	5′	TGATAGGATGTCAGAAGTCCGAAGCTCAG	RACE
	3′	GTGGAAACTTGGCAAGGTTGAAGACAC	RACE
	Forwards	CAGATGGGCAAGTTGTTTCG	Full-length amplification
	Reverse	CCATTATGTGGGCCAAGCAG	Full-length amplification
TLR2	5′	CCCTTTCTCCAACAGGCTGATTGC	RACE
	3′	ACGGAAGATGATGAACACCAAGACCTACC	RACE
	Forwards	TCTTCCCTGCCCAGCGACTC	Full-length amplification
	Reverse	GATCACCAGCAGACCAAGAC	Full-length amplification
TLR3	5′	TGGCACAATTCTGGCTCCAGTTTTGA	RACE
	3′	CTGGCCACTTCAGAAAGAAC	RACE
	Forwards	TGGAGAAGCTACTCTGATTA	Full-length amplification
	Reverse	CCCTCCCAATTTAGTCTTTA	Full-length amplification
	RT-PCR forwards	TGGGACTGTTGCCCTTTTG	qPCR
	RT-PCR reverse	CTACTTCATGCCTCACAGCACAT	qPCR
TLR4	5′	AAGGGTCCCAGCTCTCAGGTCTC	RACE
	3′	GCGGCACATCTTCTGGAGACGAC	RACE
	Forwards	CACCGAGCAAGGGAAAGAAG	Full-length amplification
	Reverse	GAATCCAGCTGCACAAGAAG	Full-length amplification
TLR5	5′	TAAGGCCATCCGACCATCAGAGGAG	RACE
	3′	ACGTCGGCTGGTTTCTCGATAAACTCTC	RACE
	Forwards	TGACCACCATCATGTCTGAG	Full-length amplification
	Reverse	TGTCCGCCATCTGTAACTTC	Full-length amplification
TLR6	5′	CAGAGAAGTGGAATGTCGTTCCAACTAC	RACE
	3′	ACAGAACTTGCACTTGGGTTGAGAG	RACE
	Forwards	AAGATCAGCCACAACCCTTT	Full-length amplification
	Reverse	GATTTCTTAAGTTGGATTTC	Full-length amplification
TLR7	5′	GACATCACAGGGCAGAGTTT	RACE
	3′	AAGAGGCTCTGTGGGAGTTC	RACE
	Forwards	CCCATCTTTGGCTGACCTTG	Full-length amplification
	Reverse	CAGAGTCCCACCTCTTCTAC	Full-length amplification
	RT-PCR forwards	CCTGGATGGAAACCAGCTTCT	qPCR
	RT-PCR reverse	GCTCAGCAGCCGTAAGCTAGA	qPCR
TLR8	5′	TGGGGAACTTCTTTCAGC	RACE
	3′	CCTCCAGTGGCCTGACAATC	RACE
	Forwards	CCTGGGCCTTCACTTGTACCTC	Full-length amplification
	Reverse	GGGTCAGTTCTTTGCATTTG	Full-length amplification
	RT-PCR forwards	CGGGAACTGTCCAAGGTGTT	qPCR
	RT-PCR reverse	TTCTGCGCAAGGCGTACA	qPCR
TLR9	5′	GCATGGCGGCCTGCACCAGGAGAG	RACE
	3′	TGACCAGGGACAACCGCCACTTCTATAAC	RACE
	Forwards	ACCAAGACCCTCAGGAGAAG	Full-length amplification
	Reverse	CCGGTCGCATTTATTGAGTG	Full-length amplification
	RT-PCR forwards	TGCAAGAACTTGACCTTCACCTT	qPCR
	RT-PCR reverse	AGAGCCCGGCGAACATCT	qPCR
TLR10	5′	GTCTGAGACCTGCCAGTGAATACCAAG	RACE
	3′	TCGCAGAGGTGAATGAAGAGTCTCAAG	RACE
	Forwards	ACAAGGGAACCAGTGCTAAA	Full-length amplification
	Reverse	GGCAACTCCAACATTATAG	Full-length amplification
TLR13	5′	TCTTCCATGTGACTTGGCTGCAAGG	RACE
	3′	GTGCCCATGAGAGGCCACTCTTCTG	RACE
	Forwards	TGCCGTTGCAGGGTGATTTC	Full-length amplification
	Reverse	CCTGCATGGTGTTTCTGTTG	Full-length amplification
18s	RT-PCR forwards	CACGGCGACTACCATCGAA	qPCR
	RT-PCR reverse	CGGCGACGACCCATTC	qPCR