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. 2008 Jan 28;32(8):890–897. doi: 10.1016/j.dci.2007.12.008

Figure 3.

Figure 3

Detection of ferret IFN-γ by a monoclonal anti-ferret IFN-γAb. Western blot analysis of recombinant ferret IFN-γ using monoclonal Abs. (LEFT PANEL) Lysates from fIFNγ-COS-7 samples were analyzed by Western blot using a monoclonal antibody established from mouse immunized by recombinant ferret IFN-γ and shown in the left panel. (RIGHT PANEL) Supernatants derived from ferret PBMC cultures stimulated with PMA plus ionomycin, were analyzed by Western blot using anti-ferret IFN-γ monoclonal antibody. Arrows at the right of each panel indicate the dimers for lower molecular weight protein bands and putative tetramer as higher molecular weight species.