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. 2008 Jan 28;32(8):890–897. doi: 10.1016/j.dci.2007.12.008

Figure 4.

Figure 4

Measurement of IFN-γ in the mitogen-stimulated ferret PBMC culture supernatants by ELISA. (A) Standard curve for ferret IFN-γ ELISA. ELISA plate well was coated with a monoclonal anti-ferret antibody generated in our laboratory. Recombinant ferret IFN-γ was sequentially diluted and loaded to the antibody-coated wells. Captured ferret IFN-γ was detected by a second monoclonal anti-ferret IFN-γ antibody generated in our laboratory conjugated to biotin, using the avidin-HRP detection method. Logarithmic dilution was used to derive a standard curve for downstream applications of the ELISA. (B) IFN-γ in mitogen stimulated ferret PBMC supernatants. ELISA utilizing the monoclonal ferret IFN-γ antibody as a capture antibody was performed on ferret PBMC cells treated with PMA, ionomycin or both. Results represent the mean values of triplicate samples.