Skip to main content
. 2008 Jan 28;32(8):890–897. doi: 10.1016/j.dci.2007.12.008

Figure 6.

Figure 6

ELISPOT assay for the IFN-γ producing cells in mitogen-stimulated ferret PBMCs. ELISPOT assay was performed in the same manner outlined for Figure 4 for capture and detection. Ferret PBMCs were plated in serial dilution and stimulated with PMA plus ionomycin for 18 h and IFN-γ secreting cells were detected by biotinylated capture antibody. The y-axis depicts the number of IFN-γ spot forming cells per well; total cells per well are indicated on the x-axis. Data shown are the average of triplicate samples.