Fig. 3.

The origin of transfer (oriT) in MOB_V1 representative plasmids. Comparison of the oriT sequences located in the pMV158, p1414 and pT181 plasmids, lined up by the position of the nic site (boldface letters). The three overlapping inverted repeats (IR1, IR2 and IR3) are depicted by arrows, and dashed lines indicate the position of those unpaired bases in the predicted secondary structures they could form. Gray background in the pMV158-oriT sequence denotes the position of a conserved repeated region. Consensus of the oriT sequences aligned in Supplementary Fig. S2 were prepared using WebLogo (version 2.8.2; Crooks et al., 2004).