Table 1.
MOBV1 plasmids.
| Plasmid name a | Nucleotide GenBank Acc. No. | Plasmid size (kb) | Relaxase GenBank Acc. No. b | Relaxase tree cluster c | Host | Replication initiator family d | sso type (position related to mob gene) e | Virulence or antibiotic/metal resistance f |
|---|---|---|---|---|---|---|---|---|
| pMV158 | NC_010096.1 | 5.541 | YP_001586274 | pMV158 | Streptococcus agalactiae | Rep_2 | ssoA (3′) + ssoU (5′) | Tc |
| pER13 | NC_002776.1 | 4.139 | NP_115336.1 | pMV158 | Streptococcus thermophilus | Rep_2 | ssoA (3′) | |
| pSMQ172 | NC_004958.1 | 4.230 | NP_862547.1 | pMV158 | Streptococcus thermophilus | Rep_2 | ssoA (3′) | |
| pGA2000 | NC_019252.1 | 4.967 | YP_006961085.1 | pMV158 | Streptococcus pyogenes | Rep_2 | ssoA (3′) | MLS(B) |
| pGB2002 | NC_015971.1 | 6.825 | YP_004831084.1 | pMV158 | Streptococcus agalactiae | Rep_2 | ssoA (3′) | MLS(B) |
| pRW35 | NC_010423.2 | 4.968 | YP_001716200.1 | pMV158 | Streptococcus pyogenes | Rep_2 | ssoA (3′) + ssoU (5′) | MLS(B) |
| pDRPIS7493 | NC_015876.1 | 4.727 | YP_004769541.1 | pMV158 | Streptococcus pseudopneumoniae | Rep_2 | ND | |
| pSSU1 | NC_002140.1 | 4.975 | NP_053061.1 | pMV158 | Streptococcus suis | Rep_2 | ssoA (3′) | |
| pYSI8 | NC_010936.1 | 4.973 | YP_001967741.1 | pYSI8 | Lactobacillus sakei | Rep_2 | ssoT (5′) | Lin |
| pK214 | NC_009751.1 | 29.871 | YP_001429536.1 | pYSI8 | Lactococcus lactis | Rep_trans and Rep_3 + L_lactis_RepB_C | ND | MEP, Strp, Chlr, Tc |
| YP_001429523.1 (MOBQ) | ||||||||
| pUR2941 | HF583290.1 | 20.876 | CCQ43999.1 | pYSI8 | Staphylococcus aureus | RepA_N + DnaB_2 and unknown and truncated | ssoA (5′) | Kan/Neo, Tc, MLS(B), Cd, Cu |
| pCPS49 | NC_019142.1 | 5.292 | YP_006958108.1 | pYSI8 | Staphylococcus aureus | Rep_2 | ssoA (3′) | PLS(A) |
| pSTE1 | NC_020237.1 | 11.951 | YP_007419104.1 | pSYI8 | Staphylococcus hyicus | Rep_trans and HTH_Hin_like and truncated | ND | Strp, MLS(B), Tc |
| YP_007419109.1 | pKKS825 | |||||||
| pKKS825 | NC_013034.2 | 14.363 | YP_003084337.1 | pKKS825 | Staphylococcus aureus | Rep_1 and HTH_Hin_like and Rep_3 | ND | Kan/Neo, Tc, Trim, PLS(A) |
| YP_003084330.1 | pKKS825 | |||||||
| YP_004679012.1 (MOBQ, truncated and fused to Rep_1) | ||||||||
| pDB2011 | NC_021513.1 | 7.641 | YP_008119849.1 | pKKS825 | Listeria innocua | Rep_1 and HTH_Hin_like | ND | MLS(B), Spec, Trim |
| pS130a | AUPT01000023.1 | 8.882 | EPZ04218.1 | pKKS825 | Staphylococcus aureus | HTH_11 | ND | Ery, Tc, Kan, Ble |
| pSCFS1 | NC_005076.1 | 17.108 | NP_899176.1 | pKKS825 | Staphylococcus sciuri | HTH_Hin_like and Rep_3 | ND | Flr/Chlr, MLS(B), Spec |
| NP_899168.1 | pNM11 | |||||||
| pLB4 | M33531.1 | Incomplete | AAA25252.1 | pLB4 | Lactobacillus plantarum | Rep_2 | ssoT (5′) | |
| pMRI_5.2 | NC_019900.1 | 5.206 | YP_007215174.1 | pLB4 | Lactobacillus plantarum | Rep_1 and Rep_2 | ssoT (3′) | |
| pLAC1 | NC_014164.1 | 3.478 | YP_003650630.1 | pLB4 | Lactobacillus acidipiscis | Rep_1 | ND | |
| pPLA4 | AF304384.2 | 8.135 | ABG23031.1 | pLB4 | Lactobacillus plantarum | Rep_3 | ND | Bacteriocin |
| pPB1 | NC_006399.1 | 2.899 | YP_138221.1 | pLB4 | Lactobacillus plantarum | Rep_2 | ssoT (5′) | |
| LkipL48 | NC_014135.1 | 3.196 | YP_003620509.1 | pLB4 | Leuconostoc kimchii | Rep_2 | ND | |
| pMBLR00 | NC_019353.1 | 3.370 | YP_006964795.1 | pLB4 | Leuconostoc mesenteroides | Rep_2 | ND | |
| pLAB1000 | M55222.1 | Incomplete | P35856.1 | pLB4 | Lactobacillus hilgardii | Rep_1 | ND | |
| pSMA23 | NC_010242.1 | 3.497 | YP_001649176.1 | pSMA23 | Lactobacillus casei | Rep_1 | ND | |
| pLC88 | U31333.1 | Incomplete | AAA74581.1 | pSMA23 | Lactococcus casei | Rep_1 | ND | |
| p141 | AB517606.1 | Incomplete | BAH97325.1 | pSMA23 | Lactobacillus plantarum | Rep_1 | ND | |
| pCD034-1 | NC_016035.1 | 3.424 | YP_004869658.1 | pSMA23 | Lactobacillus buchneri | Rep_1 | ssoT (3′) | |
| pM4 | NC_009666.2 | 3.320 | YP_001621756.1 | pSMA23 | Lactobacillus plantarum | Rep_1 | sso-new | |
| pF8801 | NC_007593 | 5.558 | YP_398641.1 | pSMA23 | Pediococcus damnosus | Rep_1 | ND | |
| pCD034-2 | NC_016034.1 | 2.707 | YP_004869655.1 | pSMA23 | Lactobacillus buchneri | Rep_2 | ssoT (3′) | |
| pG6301 | NC_019372.1 | 3.516 | YP_006965557.1 | pSMA23 | Lactobacillus plantarum | Rep_1 | ND | |
| pLB925A02 | NC_012549.1 | 3.524 | YP_002790952.1 | pSMA23 | Lactobacillus brevis | Rep_1 | ND | |
| pSD11 | NC_014919.1 | 3.225 | YP_004134615.1 | pSMA23 | Lactobacillus brevis | Rep_1 | ND | |
| pGL2 | NC_016981.1 | 4.572 | YP_005352352.1 | pGL2 | Lactococcus garvieae | Rep_2 | ssoW (3′) | Bacteriocin |
| pAMalpha1 | NC_005013.1 | 9.759 | NP_863358.1 | pAMalpha1 | Enterococcus faecalis | Rep_1 + Rep_1 and Rep_3 | ssoU (5′) | Tc |
| NP_863352.1 | pUB110 | |||||||
| Unnamed | GG670384.1 | Incomplete | EEU18290.1 | pAMalpha1 | Enterococcus faecalis | Rep_3 | ND | |
| Unnamed | GG692894.1 | Incomplete | EEU66435.1 | pAMalpha1 | Enterococcus faecalis | Rep_1 + Rep_1 and Rep_3 | ND | Tc |
| EEU66441.1 | pUB110 | |||||||
| EF62pA | NC_017314.1 | 5.143 | YP_005706998.1 | pAMalpha1 | Enterococcus faecalis | Rep_3 | ||
| pBMO2 | NC_004930.1 | 3.854 | NP_862027.1 | pBM02 | Lactococcus lactis | Rep_2 | ssoW (3′) | |
| pI4 | AF300457.1 | Incomplete | AAG28767.1 | pI4 | Bacillus coagulans | – | ssoT (5′) | Coagulin |
| pTXW | NC_013952.1 | 3.178 | YP_003517730.1 | pTXW | Lactobacillus paracasei | Rep_2 | ssoW (5′) | |
| pWCZ | NC_019669.1 | 3.078 | YP_007027014.1 | pTXW | Lactobacillus paracasei | Rep_2 | ND | |
| pLA106 | NC_004985.1 | 2.862 | NP_862697.1 | pTXW | Lactobacillus acidophilus | Rep_2 | ND | |
| pRCEID2.9 | NC_017466.1 | 2.952 | YP_005849229.1 | pTXW | Lactobacillus casei | Rep_2 | ND | |
| pT181 | NC_001393.1 | 4.439 | NP_040472.1 | pT181 | Staphylococcus aureus | Rep_trans | ssoA (3′) | |
| pSEQU3 | AVBD01000026.1 | 4.846 | ERH33926.1 | pT181 | Staphylococcus equorum | Rep_trans | ND | Tc |
| pKH17 | NC_010284.1 | 4.441 | YP_001654074.1 | pT181 | Staphylococcus aureus | Rep_trans | ND | Tc |
| pSE-12228-01 | NC_005008.1 | 4.439 | NP_863257.1 | pT181 | Staphylococcus epidermidis | Rep_trans | ND | Tc |
| pKH6 | NC_001767.1 | 4.439 | NP_053796.1 | pT181 | Staphylococcus aureus | Rep_trans | ND | Tc |
| pS0385-1 | NC_017334.1 | 5.246 | YP_005735514.1 | pT181 | Staphylococcus aureus | Rep_trans | ND | Tc |
| SAP095B | NC_013312.1 | 4.439 | YP_006937497.1 | pT181 | Staphylococcus aureus | Rep_trans | ND | Tc |
| pSBK203 | U35036.1 | Incomplete | AAA79055.1 | pT181 | Staphylococcus aureus | Rep_trans | ND | Chlr |
| pKH7 | NC_002096.1 | 4.118 | NP_052168.1 | pT181 | Staphylococcus aureus | Rep_trans | ND | Chlr |
| pS1c | AUPS01000031.1 | 3.899 | EQM91159.1 | pT181 | Staphylococcus aureus | Rep_trans | ND | |
| SAP047A | NC_013331.1 | 28.974 | YP_006938074.1 | pT181 | Staphylococcus aureus | Rep_1 and Rep_3 and RepA_N | ND | Cd, β-lac, enterotoxin G |
| pPCZ1 | NC_013539.1 | 4.738 | YP_003329162.1 | pPCZ1 | Planococcus sp. | Rep_3 | ssoA (3′) | |
| pGI1 | NC_004335.1 | 8.254 | NP_705753.1 | pGI1 | Bacillus thuringiensis | Rep_1 | ssoT (5′) | |
| pCT8513 | NC_017207.1 | 8.513 | YP_005569975.1 | pGI1 | Bacillus thuringiensis | Rep_1 | ND | |
| pB52y | AVEZ01000046.1 | 6.283 | EQM25212.1 | pGI1 | Bacillus licheniformis | Unknown | ND | |
| pBMB9741 | NC_001272.2 | 6.578 | YP_724461.1 | pGI1 | Bacillus thuringiensis | Rep_1 | ND | |
| pIS56-8 | NC_020377.1 | 8.251 | YP_007482091.1 | pGI1 | Bacillus thuringiensis | Rep_1 | ND | |
| BTB_7p | NC_018882.1 | 7.635 | YP_006931124.1 | pGI1 | Bacillus thuringiensis | Rep_1 | ND | |
| pHT7 | NC_020243.1 | 7.635 | YP_007425204.1 | pGI1 | Bacillus thuringiensis | Rep_1 | ND | |
| BTB_9p | NC_018886.1 | 8.513 | YP_006931150.1 | pGI1 | Bacillus thuringiensis | Rep_1 | ND | |
| pE33L5 | NC_007104.1 | 5.108 | YP_245942.1 | pE33L5 | Bacillus cereus | HTH_36 | ssoT (5′) | |
| pW_3 | ABCZ02000102.1 | Incomplete | EDX54234.1 | pE33L5 | Bacillus cereus | Truncated | ND | |
| pH308197_11 | NC_011340.1 | 11.567 | YP_002267516.1 | pE33L5 | Bacillus cereus | HTH_CRP | ND | |
| p1414 | NC_002075.1 | 7.949 | NP_049443.1 | p1414 | Bacillus subtilis | Rep_1 | ssoT (3′) | |
| pBamNAU-B3a | NC_022531.1 | 8.438 | YP_008628645.1 | p1414 | Bacillus amyloliquefaciens | – | ND | |
| pBA45-1 | NC_020273.1 | 8.009 | YP_007447244.1 | p1414 | Bacillus amyloliquefaciens | Rep_1 | ND | |
| pPL1 | NC_013537.1 | 6.704 | YP_003329154.1 | p1414 | Bacillus subtilis | Rep_1 and unknown | ND | |
| pTA1015 | NC_001765.1 | 5.807 | NP_053784.1 | p1414 | Bacillus subtilis | Rep_1 | ssoT (3′) | |
| pBS608 | NC_006825.1 | 6.611 | YP_195753.1 | p1414 | Bacillus subtilis | Rep_1 | ND | |
| pTA1060 | NC_001766.1 | 8.737 | NP_053788.1 | p1414 | Bacillus subtilis | Rep_1 and unknown | ssoT (3′) | |
| pBSG3 | NC_014104.1 | 8.439 | YP_003600423.1 | p1414 | Bacillus amyloliquefaciens | Rep_1 | ssoT (3′) | |
| pSD853_7.9 | NC_015392.1 | 7.860 | YP_004376195.1 | p1414 | Salmonella enterica | Rep_1 | ND | |
| pTRACA20 | NC_013279.1 | 3.780 | YP_003208332.1 | pTRACA20 | Uncultured bacterium | DNA_primase_S | ssoW (3′) | |
| pUB110 | NC_001384.1 | 4.548 | NP_040431.1 | pUB110 | Staphylococcus aureus | Rep_1 + Rep_1 | ssoU (5′) | Neo, Ble |
| pSES22 | NC_007621.1 | 4.040 | YP_415518.1 | pUB110 | Staphylococcus saprophyticus | Rep_1 | ND | MLS(B) |
| pERGB | JN970906.1 | Incomplete | AEW23141.1 | pUB110 | Staphylococcus aureus | Rep_1 and Rep_1 | ND | PLS(A), Tb, Tc, Trim |
| pTB19 | M63891.1 | Incomplete | AAA98305.1 | pUB110 | Geobacillus stearothermophilus | Rep_1 | ssoU (5′) | Tc, Ble |
| AAA98307.1 | pUB110 | |||||||
| pV7037 | HF586889.1 | Incomplete | CCQ71694.1 | pUB110 | Staphylococcus aureus | RepA_N and truncated | ND | Tc, Cd |
| pBC16 | NC_001705.1 | 4.630 | NP_043522.1 | pUB110 | Bacillus cereus | Rep_1 + Rep_1 | ssoU (5′) | Tc |
| pSWS47 | NC_022618.1 | 28.743 | YP_008719890.1 | pUB110 | Staphylococcus epidermidis | Rep_3 and truncated and truncated and RepA_N | ND | PLS(A), Kan/Neo, Tc, Trim |
| YP_008719902.1 (MOBP) | ||||||||
| pTB53 | D14852.1 | Incomplete | BAA03580.1 | pUB110 | Bacillus sp. | – | ND | |
| pIP1714 | AF015628.1 | Incomplete | AAC61672.1 | pUB110 | Staphylococcus cohnii | Rep_1 + Rep_1 | ND | PLS(A), MLS(B) |
| pNM11 | NC_019558.1 | 11.383 | YP_007016413.1 | pNM11 | Planococcus citreus | Rep_3 | ND | |
| pBS-03 | JQ394981.1 | Incomplete | AFJ49144.1 | pNM11 | Bacillus sp. | Rep_1 | ND | Flr/Chlr, Strp |
| AFJ49142.1 (MOBV, truncated) | ||||||||
| pSS-03 | NC_016054.1 | 7.122 | YP_004888092.1 | pNM11 | Staphylococcus arlettae | Rep_1 | ND | Flr/Chlr, MLS(B) |
| YP_004888090.1 (MOBV, truncated) | ||||||||
| pJ612 | NC_019186.1 | 5.048 | YP_006959664.1 | pJ612 | Haemophilus influenzae | Rep_3 | ND | β-lac |
| pA1606 | NC_019180.1 | 5.646 | YP_006959644.1 | pJ612 | Haemophilus influenzae | Rep_3 | ND | β-lac |
a
Plasmids whose relaxases were retrieved by a PSI-BLAST using MobM_pMV158 (300-N terminal residues) as a query are listed.
b
Underlined accession numbers denote those relaxase genes that are probably misannotated in the GenBank database (i.e. extended N-terminal sequence respect to that of MobM_pMV158).
c
It locates the corresponding plasmid in one of the cartooned clusters of Fig. 4, for which a prototype was selected.
d
Replication initiation protein family. When more than one, their names are separated by “and”. “+” is used for initiators that contain more than one pfam domain. Further details on replication initiation protein families can be found at http://pfam.sanger.ac.uk/.
e
Only the previously identified ssos are annotated. Most of the sso sequences span 200–300 bp and are located in close proximity to the transfer module, with the exception of plasmid pUR2941 (ssoA was mapped 7 kb upstream mob). pM4 plasmid has a new type of sso as described in (Yin et al., 2009). ND, not determined.
f
Antibiotic or metal resistance to: Tc, tetracycline; MLS(B), macrolide/lincosamide/streptogramin B; Lin, lincosamide; MEP, macrolide efflux protein; Strp, Streptomycin; Chlr, chloramphenicol; Kan, kanamycin; Neo, neomycin; Cu, copper; Cd, cadmium; PLS(A), pleuromutilins/lincosamide/streptogramin A; Trim, trimethoprim; Spec, spectinomycin; Ery: erythromycin; Ble, bleomycin; Flr, florfenicol; β-lac, beta-lactam; Tb, tobramycin.