Table 1.
Coding potential of the ECoV-NC99 genome sequence
| ORF | Encoded protein | Nucleotide position in the genome | No. of nucleotides | No. of amino acids (aa) | mRNA used for expressiona |
|---|---|---|---|---|---|
| 5′ Leader | 1–64 | 64 | |||
| 5′ UTR | 1–209 | 209 | |||
| ORF1a | pp1a | 210–13,499 | 13,290 | 4429 | 1 |
| ORF1a/b | pp1ab | 210–21,595 | 21,386 | 7128 | 1 |
| ORF2 | NS2 | 21,610–22,446 | 837 | 278 | 2 |
| ORF3 | HE | 22,458–23,729 | 1272 | 423 | 3 |
| ORF4 | S | 23,744–27,835 | 4092 | 1363 | 4 |
| ORF5 | p4.7 | 27,825–27,947 | 123 | 40 | 5 |
| ORF6 | p12.7 | 28,076–28,405 | 330 | 109 | 6 |
| ORF7 | E | 28,392–28,646 | 255 | 84 | 7 |
| ORF8 | M | 28,661–29,353 | 693 | 230 | 8 |
| ORF9a | N | 29,363–30,703 | 1341 | 446 | 9 |
| ORF9b | I | 29,424–30,044 | 621 | 206 | 9 |
| 3′ UTR | 30,704–30,992 | 289 |
a
The mRNA used for expression of each protein is derived from the Northern blotting analysis and the comparison with other group 2a coronaviruses. See the text for details.