Table 3.

Oligonucleotide primers used for RT–PCR amplification of the leader–body junction of sg mRNAs

Primer ID	Position	Sequence (5′–3′)	Use
22813N	22,792–22,813	GCGTTATCACCAGAAGCGGTGC	Reverse transcription for mRNA2 (NS2) and reverse primer for mRNA3 (HE) PCR
25095N	25,076–25,095	CGCCTATTCCAGGCAGAAGG	Reverse transcription for mRNA3 (HE) and mRNA4 (S)
29101N	29,078–29,101	GGCAGTAAGAGTATGATGGTCCTC	Reverse transcription for mRNA5 (p4.7), mRNA6 (p12.7) and mRNA7 (E)
30945N	30,921–30,945	CTGGGTGGTAACTTAACATGCTGGC	Reverse transcription for mRNA8 (M) and mRNA9 (N)
1P	1–21	GATTGTGAGCGAATTGCGTGC	Forward primer for all sg mRNA PCR
21982N	21,958–21,982	GACGGGACTGACCAACTACACAACC	Reverse primer for mRNA2 (NS2) PCR
24283N	24,262–24,283	GCGTGGTGACCCAATACCACTG	Reverse primer for mRNA4 (S) PCR
28100N	28,078–28,100	TCCTCTCAGGTCTCCAGATGTCC	Reverse primer for mRNA5 (p4.7) PCR
28334N	28,312–28,334	CAGCCTCCTCTATAGTATTGGCG	Reverse primer for mRNA6 (p12.7) PCR
28641N	28,617–28,641	CGTCATCCACATTAAGGACTGGTGG	Reverse primer for mRNA7 (E) PCR
29016N	28,992–29,016	GGGTTGAAACTCCACCAACTACCAG	Reverse primer for mRNA8 (M) PCR
29710N	29,691–29,710	GCGTTGATTGCCATCGGCTG	Reverse primer for mRNA9 (N) PCR