Fig. 4.
FCoV N gene, TNF-alpha, and fAPN mRNA expression levels in macrophages of FIP and SPF cats. Alveolar macrophages (2 × 106 cells) were collected from FIP and SPF cats, and the FCoV N gene (A), TNF-alpha mRNA (B), and fAPN mRNA (C) were detected by RT–PCR. The FCoV N gene, TNF-alpha mRNA, and fAPN mRNA were quantitatively analyzed in terms of the relative density value to the mRNA for the housekeeping gene GAPDH. n = 7, N.D.: not detected.