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. 2008 Mar 18;374(2):240–248. doi: 10.1016/j.virol.2008.02.016

Fig. 3.

Fig. 3

Detection of autophagosome in DV2-infected Huh7 and mouse MEF cells under TEM. The cells were fixed at 36 h post DV2 infection and observed under TEM. (A) Normal Huh7 cells without infection and treatment; (B) Huh7 cells were infected with DV2. The dot-line box encompassing the autophagosome was further enlarged in the inlet to show the outer- and inner-membrane (white-arrow: outer-membrane and black-arrow: inner-membrane). (C) Huh7 cells were infected with DV2 plus 10 mM 3-MA treatment. (D) ATG5+/+ MEF cells were infected with DV2. (E) ATG5−/− MEF cells were infected with DV2. (F) ATG5+/+ MEF cells were infected with DV2 plus 3-MA treatment. Arrowheads: virus-like particle. Arrow: autophagosome vesicle. Cy: cytoplasm; ER: endoplasmic reticulum; G: Golgi apparatus; N: nucleus. (G) Huh7 cells infected with DV2 for 36 h were fixed and stained with anti-LC3 antibody, and visualized by a secondary antibody coupled to 5-nm gold particles. The arrow indicates gold particles representing LC3, and the arrowhead indicates virus-particle (20–40 nm).