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. 2008 Mar 18;374(2):240–248. doi: 10.1016/j.virol.2008.02.016

Fig. 4.

Fig. 4

Determination of DV2 titer in Huh7 and MEF cells by plaque assay and flow-cytometry. (A) Extracellular viral titers in DV2 infected Huh7 cells with or without 3-MA or rapamycin were determined by plaque assay at the indicated times. Moreover, the extracellular viral titers in DV2 infected Huh7 cells combined with various dosages of Rapamycin (B) or 3-MA (C) were determined by plaque assay at 36 h p.i. (D) The intracellular DV-NS1 expression levels in DV2 infected Huh7 cells were determined using anti-DV-NS1 antibody labeling followed by flow-cytometry analysis. FL1-NS1: intensity of NS1 fluorescence; Event count: cell number; GM: Mean fluorescence of NS1 expressing cells. (E) DV2 titers in ATG5 wild-type and ATG5 knockout MEF cells were measured by plaque assay at indicated times. Each data point is representative of the results of three independent experiments. ATG5-WT: wild-type MEF cells; ATG5-KO: ATG5 knockout MEF cells; ⁎⁎⁎: means p < 0.05.