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Detection of PEDV RNA in virus suspension recovered from feces and all virus cultures from passage 1 to 5 by nested RT–PCR assays. 412-bp PCR fragments (sequence confirmed) could be observed. Lanes 1 and 2, PEDV of virus suspension purified from feces and negative control. Lanes 3 to 12, 5 serial passaged PEDV in pAPN-expressing MDCK cells and pAPN-deficient MDCK cells as control groups.
