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Vero cells were infected with serially diluted supernatants collected from PEDV-infected mock- and pAPN-transfected MDCK cells at serial passage 5. Inoculation with the serially diluted supernatant of pAPN-transfected cells corresponds to the decrease of cytotoxicity. However, infection with the serially diluted supernatant of pcDNA3.1-transfected cells merely resulted in minimal loss of viability without gradient change.
