Fig. 15.

Oncolytic effect of magnetic Ad520 in multidrug-resistant 181RDB-fLuc Cells. (a) IC₅₀ for the oncolysis of the 181RDB-fLuc cells with virus alone or with magnetic virus complexes prepared at different nanoparticle-to-virus particle ratios versus time postinfection in cell culture medium containing 7.5% FCS with positioning on the magnetic plate for 30 min after adding the virus or magnetic complexes. (c) Oncolytic effect of Ad520 or Ad520–PEI-Mag2 complexes at a ratio of 5 fg of Fe/VP in 181RDB-fLuc cells were plotted against the internalized virus dose assessed from the experimental curves on virus internalization shown in Fig. 9. IC₅₀ was calculated in terms of the internalized pfu/cell for both the virus and its magnetic complexes.

Reproduced with permission from ACS Publications: Molecular Pharmaceutics [55].