Table 3.
Characteristics of selected siRNA magnetic complexes. Mf stands for Metafectene (Biontex).
| Complex | Iron-to-siRNA w/w ratio | ξ-Potential (mV) | Mean hydrated diameter D (nm) | Efficient velocity in magnetic fieldsa υz (μm/s) | Average magnetic moment of the complex Ma (10− 16 A m2) | Number of magnetic particles in a complexN = M/meff |
|---|---|---|---|---|---|---|
| Duplexes | ||||||
| PEI/siRNA | – | + 15.2 ± 1.8 | 413 ± 190 | – | – | |
| Mf/siRNA | – | + 36.1 ± 9.7 | 283 ± 133 | – | – | |
| PEI–Mag2/siRNA | 1:1 | − 14.0 ± 0.8 | 685 ± 242 | 1.2 | 17.2 | 20,483 |
| PEI-Mag2/siRNA | 2:1 | − 10.1 ± 1.2 | 736 | 1.96 | 30.2 | 35,946 |
| Triplexes | ||||||
| PEI-Mag2/PEI/siRNA | 0.5:1 | + 2.0 ± 4 | 394 ± 70 | 1.19 | 9.5 | 11,290 |
| PalD1-Mag1/PEI/siRNA | 0.5:1 | + 7.2 ± 1.5 | 370 ± 115 | 1.49 | 11.6 | 15,895 |
| PEI-Mag2/Mf/siRNA | 0.5:1 | 36.4 ± 3.8 | 210 ± 86 | 0.86 | 3.8 | 4500 |
| PalD1-Mag1/Mf/siRNA | 0.5:1 | + 12 ± 6.3 | 326 ± 175 | 0.72 | 30.2 | 12,181 |
a Determined at < B > = 213 mT and gradient B of 4 T/m.
Reproduced with permission from Humana Press: Methods in Molecular Biology [76].