Table 4.
Physicochemical characteristics of the complexes of adenovirus Ad520 with PEI-Mag2 magnetic nanoparticles.
| Nanoparticle-to-virus ratio at complex preparation |
Zeta potential, ξ (mV) |
|||||||
|---|---|---|---|---|---|---|---|---|
| fg of Fe/VP | MNP/VP | Mean hydrodynamic diameter, Dh (nm)a | Polydispersity index, Pla | In OptiMEM or PBSa | In cell culture mediuma, b | av velocity, υz (μm/s)c, d | Magnetic moment, M (10− 16 A m2)c | No. of MNPs associated with complex |
| Complexes assembled in OptiMEM at virus concentration of 5.4 × 108 VP/ml | ||||||||
| 0 | 0 | 159 ± 12 | 0.46 | − 18.0 ± 3.7 | − 5.6 ± 2.8 | |||
| 2.5 | 1736 | 438 ± 219 | 0.50 | + 5.0 ± 0.4 | − 9.8 ± 1.6 | 1.41 | 12.9 | 14,961 |
| 5 | 3472 | 288 ± 65 | 0.25 | + 15.1 ± 1.7 | − 9.2 ± 0.7 | 0.87 | 4.1 | 4778 |
| 10 | 6944 | 206 ± 36 | 0.18 | + 18.9 ± 1.2 | − 8.3 ± 1.1 | 0.86 | 3.7 | 4283 |
| 20 | 13,889 | 192 ± 9 | 0.11 | + 21.5 ± 1.7 | − 6.3 ± 1.1 | 0.95 | 3.8 | 4407 |
| 40 | 27,778 | 298 ± 141 | 0.26 | + 21.9 ± 3.2 | + 0.3 ± 3.7 | 1.02 | 6.4 | 7371 |
| Complexes assembled in PBS at virus concentration of 2.1 × 109 VP/ml | ||||||||
| 0 | 0 | 200 ± 50 | 0.49 | − 9.1 ± 1.3 | − 6.0 ± 1.2 | |||
| 0.625 | 434 | 354 ± 44 | 0.27 | − 3.8 ± 0.6 | − 8.8 ± 0.8 | 0.22 | 5.5 | 6404 |
| 1.25 | 868 | 666 ± 83 | 0.26 | + 3.2 ± 0.8 | − 9.2 ± 0.4 | 0.35 | 4.9 | 5689 |
| 2.5 | 1736 | 186 ± 23 | 0.25 | + 16.1 ± 1.0 | − 7.3 ± 0.2 | 0.39 | 2.5 | 2847 |
| 5 | 3472 | 220 ± 55 | 0.24 | + 17.1 ± 0.9 | − 8.0 ± 0.6 | 0.55 | 3.1 | 3612 |
| 10 | 6944 | 246 ± 29 | 0.23 | + 14.7 ± 0.8 | − 7.1 ± 0.3 | 0.85 | 3.9 | 4484 |
| 20 | 13,889 | 249 ± 76 | 0.25 | + 17.4 ± 1.1 | − 6.5 ± 0.4 | 0.95 | 5.9 | 6844 |
| 40 | 27,778 | 319 ± 56 | 0.27 | + 16.9 ± 0.6 | − 5.3 ± 0.1 | 1.39 | 3.7 | 4297 |
a
Mean ± SD (n = 50).
b
Measured after 4-fold dilution of the complex with full cell culture medium containing 10% FCS.
c
Measured at B-average of 213 mT
d
Measured at ∇B-average of 4 T/m.
Reproduced with permission from ACS Publications: Molecular Pharmaceutics [55].