. 2012 Feb 15;30(7):367–370. doi: 10.1016/j.eimc.2011.12.001

Table 1.

Primers used for detection of respiratory viruses using two nested multiplex RT-PCRs.

Virus	Step	Name	Sequence (5′-3′)	Sense	Amplicon size (pb)
IA, IB	RT-PCR A	FluAC1^a	GAACTCRTCCYWWATSWCAAWGRRGARAT	+	IA: 721 IB: 991
		FluB1^a	ACAGAGATAAAGAAGAGCGTCTACAA	+
		FluABC2^a	ATKGCGCWYRAYAMWCTYARRTCTTCAWAKGC	−
	Nested PCR A	FluAB3^a	GATCAAGTGAKMGRRAGYMGRAAYCCIGG	+	IA: 301 IB: 226
		FluAC4^a	TCTTCAWATGCARSWSMAWKGCATGCCATC	−
		FluB4^a	CTTAATATGGAAACAGGTGTTGCCATATT	−

VSR A, VSR B	RT-PCR A	RSVAB1^a	ATGGAGYTGCYRATCCWCARRRCAARTGCAAT	+	737
	RT-PCR A	RSVAB2^a	AGGTGTWGTTACACCTGCATTRACACTRAATTC	−	737
	Nested PCR A	RSVA3^a	TTATACACTCAACAATRCCAAAAAWACC	+	VSR A: 363 VSR B: 611
		RSVA4^a	ATCTTCCTAACTCTTGCTRTTAATGCATTG	+
		RSVB3^a	AAATTCCCTGGTAATCTCTAGTAGTCTGT	−
		RSVB4^a	GATGCGACAGCTCTGTTGATTTACTATG	−

Rhinovirus	RT-PCR A	Rino-E1^b	CTCCGGCCCCTGAATRYGGCTAA	+	639
	RT-PCR A	Rino-E2^b	TCIGGIARYTTCCASYACCAICC	−	639
	Nested PCR A	Rino-I3^b	ACCRASTACTTTGGGTRWCCGTG	+	110
	Nested PCR A	Rino-I4^b	CTGTGTTGAWACYTGAGCICCCA	−	110

Metapneumovirus	RT-PCR B	Meta-E1	CAAGGTGCAGCAATGTCTGT	+	357
	RT-PCR B	Meta-E2	TGCAATTTTGGCCTGTGTTA	−	357
	Nested PCR B	Meta-I3	AACCATACGGGATGGTATCAA	+	200
	Nested PCR B	Meta-I4	GCTTGGTCTGCTTCACTGCT	−	200

Parainfluenzavirus	RT-PCR B	Para-E1	TGGMTCYGGATCMTTACCAAT	+	358
	RT-PCR B	Para-E2	CAGATTSAYTGATATTGWGTTSGGT	−	358
	Nested PCR B	Para-I3A	CTGCATTGATCTCAAAATAACT	+	164
		Para-I3B	TGCAACTAAACTGGACATAGAA	+
		Para-I4	GGRAGACATTGAGGWGCWAGA	−

Coronavirus	RT-PCR B	Coro1N	TCCACAATTTGCTGAGCTTG	+	250
	RT-PCR B	Coro2N	TTCAGCAGTTGCAGGTGAAG	−	250
	Nested PCR B	Coro3N	TTCCAAAGAGTCAGGCAACA	+	148
	Nested PCR B	Coro4N	TGCACTAGGGTTAAGAAGAGGA	−	148

Primers taken from Coiras et al.³ FluAC1 and FluAB3 were further modified.

Primers taken from Coiras et al.⁴