Table 1.
Virus | Step | Name | Sequence (5′-3′) | Sense | Amplicon size (pb) |
---|---|---|---|---|---|
IA, IB | RT-PCR A | FluAC1a | GAACTCRTCCYWWATSWCAAWGRRGARAT | + | IA: 721 IB: 991 |
FluB1a | ACAGAGATAAAGAAGAGCGTCTACAA | + | |||
FluABC2a | ATKGCGCWYRAYAMWCTYARRTCTTCAWAKGC | − | |||
Nested PCR A | FluAB3a | GATCAAGTGAKMGRRAGYMGRAAYCCIGG | + | IA: 301 IB: 226 |
|
FluAC4a | TCTTCAWATGCARSWSMAWKGCATGCCATC | − | |||
FluB4a | CTTAATATGGAAACAGGTGTTGCCATATT | − | |||
VSR A, VSR B | RT-PCR A | RSVAB1a | ATGGAGYTGCYRATCCWCARRRCAARTGCAAT | + | 737 |
RSVAB2a | AGGTGTWGTTACACCTGCATTRACACTRAATTC | − | |||
Nested PCR A | RSVA3a | TTATACACTCAACAATRCCAAAAAWACC | + | VSR A: 363 VSR B: 611 |
|
RSVA4a | ATCTTCCTAACTCTTGCTRTTAATGCATTG | + | |||
RSVB3a | AAATTCCCTGGTAATCTCTAGTAGTCTGT | − | |||
RSVB4a | GATGCGACAGCTCTGTTGATTTACTATG | − | |||
Rhinovirus | RT-PCR A | Rino-E1b | CTCCGGCCCCTGAATRYGGCTAA | + | 639 |
Rino-E2b | TCIGGIARYTTCCASYACCAICC | − | |||
Nested PCR A | Rino-I3b | ACCRASTACTTTGGGTRWCCGTG | + | 110 | |
Rino-I4b | CTGTGTTGAWACYTGAGCICCCA | − | |||
Metapneumovirus | RT-PCR B | Meta-E1 | CAAGGTGCAGCAATGTCTGT | + | 357 |
Meta-E2 | TGCAATTTTGGCCTGTGTTA | − | |||
Nested PCR B | Meta-I3 | AACCATACGGGATGGTATCAA | + | 200 | |
Meta-I4 | GCTTGGTCTGCTTCACTGCT | − | |||
Parainfluenzavirus | RT-PCR B | Para-E1 | TGGMTCYGGATCMTTACCAAT | + | 358 |
Para-E2 | CAGATTSAYTGATATTGWGTTSGGT | − | |||
Nested PCR B | Para-I3A | CTGCATTGATCTCAAAATAACT | + | 164 | |
Para-I3B | TGCAACTAAACTGGACATAGAA | + | |||
Para-I4 | GGRAGACATTGAGGWGCWAGA | − | |||
Coronavirus | RT-PCR B | Coro1N | TCCACAATTTGCTGAGCTTG | + | 250 |
Coro2N | TTCAGCAGTTGCAGGTGAAG | − | |||
Nested PCR B | Coro3N | TTCCAAAGAGTCAGGCAACA | + | 148 | |
Coro4N | TGCACTAGGGTTAAGAAGAGGA | − |