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. 2007 Jun 22;367(1):41–50. doi: 10.1016/j.virol.2007.05.013

Fig. 2.

Fig. 2

Protein purification of the helicase-like domain of BaMV replicase. The E. coli-expressed enzymes were purified through immobilized metal affinity and anionic exchange chromatography as described under Materials and methods. Each of the purified enzymes was resolved on SDS–PAGE (10%) and stained by Coomassie blue.