Fig. 4.

RNA 5′-triphosphatase reaction catalyzed by the wild-type enzyme. (A) Time course of reactions in 5 μl solution that contained 0.1 pmol purified protein, 1 μM 5′-[γ-³²P]RNA and other components as described under Materials and methods. (B) Reactions carried out for 1 min in 3 μl solution that contained 240 pmol (80 μM) 5′-[γ-³²P]RNA, indicated amounts of enzyme and other components as described under Materials and methods.