. 2007 Jun 22;367(1):41–50. doi: 10.1016/j.virol.2007.05.013

Table 1.

Kinetic parameters of NTP hydrolysis catalyzed by wild-type helicase-like domain of BaMV replicase

Substrate	K_m (μM)	k_cat (s⁻ ¹)	k_cat/K_m (μM⁻ ¹ s⁻ ¹)
ATP	150	67	0.45
GTP	210	35	0.17
UTP	220	24	0.11
CTP	330	30	0.09

The enzymatic activity was determined at 20 °C by enzyme-coupled assay in 1 ml solution that contained 10 pmol enzyme, 0.1 to 3 mM NTP and other buffer components as described under Materials and methods except that the amounts of pyruvate kinase were increased up to 50, 75 and 300 U for GTPase, UTPase and CTPase assay, respectively, to assure the rate of NTP hydrolysis being the limiting step within the coupling reaction.