Table 4.

Unique amino acid residues observed in structural and nonstructural proteins encoded by the 3′ end of the bubaline CoV genome in comparison with other ruminant CoVs

CoV strain	32 kDaa			HE			S
	205	217	275	108	158	210	143	147	151	157	257	318	328	525	608	785	805	828	888	909	927	1260
BuCoV-179/07-11	K	N	F	H	P	N	Y	F	I	V	N	T	F	Y	G	K	D	T	K	R	A	N
BCoV-Mebus	M	K	V	Y	S	K	H	L	L	I	T	I	L	H	D	N	E	A	N	K	S	D
BCoV-ENT	M	K	V	Y	S	K	H	L	L	I	T	I	L	H	D	N	E	A	N	K	S	D
BCoV-Quebec	M	K	V	Y	S	K	H	L	L	I	T	I	L	H	D	N	E	A	N	K	S	D
BCoV-DB2	M	K	V	Y	S	K	H	L	L	I	T	I	L	H	D	N	E	A	N	K	S	H
GiCoV-US/OH3/2003	M	K	V	Y	S	K	H	L	L	I	T	I	L	H	D	N	E	A	N	K	S	D
ACoV	M	K	V	Y	S	K	H	L	L	I	T	I	L	H	D	N	E	A	N	K	S	D
SACoV-US/OH1/2003	M	K	V	Y	S	K	H	L	L	I	T	I	L	H	D	N	E	A	N	K	S	D
CRCoV-4182	M	K	V	Y	S	K	H	L	L	I	T	I	L	Y	G	N	E	V	N	K	S	D
HECV-4408	NA	NA	NA	Y	S	K	H	L	L	I	S	I	L	H	G	N	E	A	N	R	A	D
HCoV-OC43	M	K	V	Y	A	R	R	S	V	V	T	I	L	D	D	N	E	A	N	K	S	D
PHEV-VW572	M	NP	NP	Y	P	K	H	I	L	I	S	V	L	T	D	N	E	T	N	R	S	D

CoV strain	4.9 kDab			4.8 kDab							12.7 kDa	M		N		I
	24	32	40	2	9	16	28	29	40	41	70	58	158	152	354	56	116	122	132
BuCoV-179/07-11	P	A	T	Q	D	K	G	F	N	W	F	L	S	H	S	K	I	S	M
BCoV-Mebus	L	E	M	P	G	M	C	S	T	C	S	I	G	Q	N	R	T	L	R
BCoV-ENT	L	NP	NP	P	G	M	V	S	T	C	S	I	G	Q	N	R	T	L	R
BCoV-Quebec	L	E	M	NP	G	M	C	S	T	C	S	I	G	Q	N	R	T	L	R
BCoV-DB2	L	E	M	P	G	M	V	S	T	C	S	I	G	Q	N	R	T	L	R
GiCoV-US/OH3/2003	L	NP	NP	P	V	M	V	S	NP	NP	S	I	G	Q	N	R	T	L	R
ACoV	L	NP	NP	P	V	M	V	S	T	S	S	I	G	Q	N	R	T	L	R
SACoV-US/OH1/2003	L	NP	NP	P	V	M	V	S	NP	NP	S	I	G	Q	N	R	T	L	R
CRCoV-4182	NP	NP	NP	NP	NP	NP	NP	NP	NP	NP	S	I	G	Q	N	R	T	L	R
HECV-4408	L	E	T	Q	D	M	G	S	N	W	S	L	G	Q	N	R	T	L	R
HCoV-OC43	NP	NP	NP	NP	NP	NP	NP	NP	NP	NP	S	I	G	Q	N	NP	T	L	R
PHEV-VW572	F	NP	NP	P	NP	NP	NP	NP	NP	NP	S	I	G	Q	N	R	T	L	R

NA, not available; NP, not present.

^aNsp 32 kDa of HECV-4408 is not available in the GenBank database, whereas it is truncated in PHEV-VW572.

^bNsp 4.9 kDa and 4.8 kDa are not present in the CRCoV-4182 and HCoV-OC43 genomes. Nsp 4.9 kDa is truncated in PHEV-VW572.