Table 6.

Primers used for RT-PCR amplification and sequence analysis

Primer	Sequence 5′ to 3′	Sense	Positiona	Amplicon size (bp)
BCV-22001F	TAGACTTGAAATAGTTAAGCTTGGTG	+	22,001–22,026	893
BCV-22894R	AAATTAGCTTCACGAGCTATATATGC	−	22,868–22,893
BCV-22769F	TATCGCAGCCTTACTTTTGTTAATG	+	22,769–22,793	527
BCV-23295R	CGAAAATAACAGTACGGGGGTTGACA	−	23,270–23,295
BCV-23112F	TACCCTCTGGTAATTATTTAGCCATTTCA	+	23,112–23,140	776
BCV-23887R	TTCCCTTCAGTGCCATATTACGATATGT	−	23,860–23,887
BCV-23510F	TATGATCCGCTACCAATTATTTTGCTTGGCA	+	23,510–23,540	817
BCV-24326R	ACAACACCAGTGTCTGTAAAATATGCA	−	24,300–24,326
BCV-24182F	TAGAACTATGGCATTGGGATACAGGTGTTG	+	24,182–24,211	1254
BCV-25435R	TACACCTATCCCCTTGTAAACAAGAGTA	−	25,409–25,435
BCV-25301F	ACTTAGTTGGCATAGGTGAGCACTGTTC	+	25,301–25,328	851
BCV-26151R	ACATGCTACATAATCACCACAGACAA	−	26,126–26,151
BCV-26028F	TTTGTATGAAATTCAAATACCTTCAGAG	+	26,028–26,055	877
BCV-26904R	GTCTATCTGAGCTTGCGCTTCAAGAGCA	−	26,877–26,904
BCV-26760F	TAAAATTCAAGCTGTTGTTAATGCAAAT	+	26,760–26,787	1059
BCV-27818R	GCTCGACCTAAATGGGTCTTATAATTAGA	−	27,791–27,818
BCV-27667F	GGTGGTTGTTGTGATGATTATACTGGACA	+	27,667–27,695	853
BCV-28519R	ACTACAACTATTATAACCAATAAACACAT	−	28,491–28,519
BCV-28380F	TCTTAGCTGTTGACTTTATTACCTGG	+	28,380–28,405	919
BCV-29298R	ACATAAACAGCAAAACCACTAGTATCGCC	−	29,270–29,298
BCV-29160F	AAGGTATAAAACTAGGTACTGGCTAT	+	29,160–29,185	876
BCV-30035R	TGCGCGATCCTGCACTAGAGGCTCTAC	−	30,009–30,035
BCV-29900F	GAGGCTATTCCGACTAGGTTTCCGCCT	+	29,900–29,926	827
BCV-30726R	GTGTCTTCAGTAAAGGGCTCATCCATC	−	30,700–30,726
BCV-30610F	TGATAATATAAGTGTTGCAGCGCCCAAA	+	30,610–30,637	419
BCV-31028R	GTGATTCTTCCAATTGGCCATAATT	−	31,004–31,028

^aPrimers position is referred to the sequence of BCoV strain ENT (accession number AF391541).