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. 2007 Aug 10;368(2):296–308. doi: 10.1016/j.virol.2007.05.032

Fig. 2.

Fig. 2

Characterization of intracellular and extracellular rTGEV-ΔE virus in BHK-pAPN-E+ and E− cells. (A) Assembly characterization of rTGEV-ΔE in BHK-pAPN-E+ and E− cells by electron microscopy. Panels show sections from rTGEV-ΔE infected BHK-pAPN-E+ and E− cells at 24 h p.i. Scale bars, 300 nm. (B) The presence of TGEV proteins in supernatants was analyzed by SDS-PAGE using 5 to 20% gradient gels and silver staining. The arrows to the right of the panel indicate the positions of TGEV structural proteins. (C) Electron micrographs of virions from supernatants of rTGEV-ΔE infected BHK-pAPN-E+ and E− cells concentrated 200-fold, and stained with 2% uranyl acetate are shown. Scale bar, 100 nm.