Fig. 1.

Effect of cholesterol depletion and replenishment on virus infection. (A) Vero CCL-81 cells were treated with different mβCD concentrations. After removal of mβCD by three washing steps, cells were infected by VSV-ΔG-S (white bars) and the control viruses VSVΔG-G (black bars) and IBV (hatched bars). At 8 h p.i. cells were fixed. Infection was determined by counting eGFP-expressing (VSV-ΔG viruses) or by immunostaining (IBV). (B) Untreated and mβCD-treated Vero cells were incubated in the absence (−) or presence (+) of water-soluble cholesterol diluted in medium followed by infection with VSV-ΔG pseudotype viruses. Infectivity rates were determined as described for 1A. Each bar represents the mean ± standard deviation of 3 independent experiments.