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ELISPOT assay to measure the production of IFN-γ by splenocytes (black bars) of an IBV- and NDV-vaccinated White Leghorn chicken and by cow PBMC (white bars) in triplicate after in vitro stimulation with ConA (10 μg/ml), PMA (100 ng/ml) and ionomycin (500 ng/ml), NDV (105 EID50), REV (105 EID50), IBV M41 (105 EID50), or UV-inactivated IBV M41 (105 EID50). Bars marked with ⁎ are significantly different (p < 0.05).
