Fig. 6.

Real-time quantification of ChIFN-γ mRNA expression by splenocytes collected 7 days after IBV infection from four IBV-infected and four IBV-uninfected 5 wk old commercial broiler chickens, 6 h after stimulation with PMA (100 ng/ml) and ionomycin (500 ng/ml), or IBV M41 (10⁵ EID₅₀). Bars represent the mean fold increase (plus standard deviation) in ChIFN-γ mRNA production compared to unstimulated splenocytes. All groups are significantly different from the unstimulated splenocytes (⁎p < 0.05; ⁎⁎p < 0.01).