Fig. 6.

FRAP analysis of EGFP, EGFP-N, EGFP-Rev, EGFP-ORF57, EGFP-ORF57Δ_NoLS/Rev and EGFP-NΔ_NoLS/ORF57. Images were sampled pre-bleach and post-bleach five times a second for 60 s. EGFP-fusion proteins are false coloured to show their concentration using the ‘rainbow’ feature on the Zeiss LSM imaging software, where red is high intensity and blue is low intensity. The experiment was repeated five times and a representative image from each cell series is shown for pre-bleach, immediately post-bleach (0 s), 10, 20, 30 and 60 s post-bleach. The bleach area in the nucleolus is denoted in the immediate post-bleach image (0 s), and the width of the marker is 15 μm.