Fig. 8.

FLIP analysis of EGFP-nucleolin, EGFP-fibrillarin and EGFP-B23.1. One nucleolus was continuously photo-bleached (indicated by an arrow) and images were sampled at 0, 10, 20, 30 and 60 s. EGFP-fusion proteins are false coloured to show their concentration using the ‘rainbow’ feature on the Zeiss LSM imaging software, where red is high intensity and blue is low intensity. The experiment was repeated five times and a representative image from each cell series is shown. The width of the marker is 10 μm.