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. 2008 Mar 14;375(2):391–400. doi: 10.1016/j.virol.2008.02.019

Fig. 1.

Fig. 1

Endocytosis of EB2 in different cell lines. EB2-expressing cells (Vero, MDCK, PAEC, and HeLa) were incubated with EphB4/Fc for 1 h at 37 °C to allow binding and endocytosis to proceed. Surface-remained EphB4/Fc was stained with a rhodamine-conjugated anti-human IgG antibody (surface). After fixation and permeabilization, internalized EphB4/Fc was detected by a FITC-conjugated secondary antibody (intracellular).