Fig. 5.

Effect of sucrose and NH₄Cl treatment on influenza virus entry. Vero cells were infected with FPV at a MOI of 0.01. Cells were either left untreated (control) or incubated with the indicated inhibitors for 1 h prior infection and for 1 h during virus adsorption and entry. After washings to remove the inhibitors and input virus, residual virus attached to the cell surface was inactivated by acidic pH treatment. At 16 h p.i., cells were fixed and stained with a FPV-specific monoclonal antibody. Primary antibodies were detected with rhodamine-conjugated secondary antibodies and nuclei were visualized by DAPI staining.