Fig. 2.

Replication of RCNMV RNA1 mutants deficient in expression of either p27 or p88 by supplying their lacking component in cowpea protoplasts. Cowpea protoplasts were inoculated with plasmids, and accumulation of progeny viral RNAs were analyzed by Northern blotting after 24 h of incubation. (A) Accumulations of RNA1 mutants in single and mixed inoculation. (B) Accumulations of RNA1 mutants when p27 or p88 was supplied from protein expression vectors. pUBRC2 was also inoculated to assess and to confirm the expression of p27 and p88. (C) Accumulations of RNA1 mutants with the coat protein (CP) gene deleted. The combination of plasmids used for the inoculation is indicated above each lane.