Table 1.
Determination of interactions among CymMV encoded triple gene block proteins by yeast two-hybrid assay.
| AD fusiona | BD fusiona | Trp− and Leu− mediumb | Trp−, Leu−, His− and Ade− mediumc | Histochemical assayd (X-α-gal) |
|---|---|---|---|---|
| T-antigene | p53e | +++ | +++ | +++ |
| TGBp1 | ⁎ | +++ | − | NA |
| TGBp2 | ⁎ | +++ | − | NA |
| TGBp3 | ⁎ | +++ | − | NA |
| CP | ⁎ | +++ | − | NA |
| ⁎ | TGBp1 | +++ | − | NA |
| ⁎ | TGBp2 | +++ | − | NA |
| ⁎ | TGBp3 | +++ | − | NA |
| ⁎ | CP | +++ | − | NA |
| TGBp1 | TGBp1 | +++ | +++ | +++ |
| TGBp1 | TGBp2 | +++ | − | NA |
| TGBp1 | TGBp3 | +++ | − | NA |
| TGBp3 | TGBp1 | +++ | − | NA |
| TGBp3 | TGBp3 | +++ | − | NA |
| CP | CP | +++ | +++ | +++ |
a
“⁎”, plasmid expression AD domain (pGADT7) and BD domain (pGBKT7) are added.
b
“+”, yeast colonies that grew on SD medium (Lacking Trp and Leu). “−”, yeast colonies that did not grow on SD medium (lacking Trp and Leu).
c
“+”, yeast colonies that grew on SD medium (Lacking Trp, Leu, His and Ade). “−”, yeast colonies that did not grow on SD medium (Lacking Trp, Leu, His and Ade).
d
“+”, yeast colonies that were blue. “NA” indicates yeast colonies did not grow and no blue histochemical stain was observed.
e
Plasmids containing T-antigen-AD and murine p53-BD were provided by the manufactory and used as positive controls.