Fig. 5.

Potential crucial amino acids for binding of mAb with RBD are predicted. Underlined letters represent amino acids recognized by the mAbs in dot-blot experiments. Capital letters represent amino acids that were predicted by computer model to be potentially important for the binding of the mAb with the RBD. Combining the results of the two methods, amino acids VAD, VL, FF, WN and RNI were selected to be the most important amino acids determined in a site-directed mutagenesis experiment.