Table 2.

Primers for site-directed mutagenesis; construction of RBD-Fc expressing plasmids

aa. residues mutated	Primer sequences
FF → AA	SP: TAC AAC TCA ACA GCT GCT TCA ACC TTT AAG
	AS: CTT AAA GGT TGA AGC AGC TGT TGA GTT GTA
WN → AA	SP: TGT GTC CTT GCT GCT GCT ACT AGG AAC ATT
	AS: AAT GTT CCT AGT AGC AGC AGC AAG GAC ACA
VL → AA	SP: GCT GAT TAC TCT GCT GCT TAC AAC TCA ACA
	AS: TGT TGA GTT GTA AGC AGC AGA GTA ATC AGC
VAD → AAA	SP: AAA ATT TCT AAT TGT GCT GCT GCT TAC TCT GTG CTC TAC
	AS: GTA GAG CAC AGA GTA AGC AGC AGC ACA ATT AGA AAT TTT
RNI → AAA	SP: CTT GCT TGG AAT ACT GCT GCT GCT GAT GCT ACT TCA ACT
	AS: AGT TGA AGT AGC ATC AGCAGC AGC AGT ATT CCA AGC AAG

The candidate amino acid residues were mutated to alanines within the primers designed for mutation. SP: sense primer. AS: anti-sense primer.