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. 2009 Mar 21;387(2):402–413. doi: 10.1016/j.virol.2009.02.021

Fig. 6.

Fig. 6

Association of 8ab with ATF6. (A) Indirect immunofluorescence analysis of 3XFLAG-ATF6S1P−. HeLa cells were transiently co-transfected with 3XFLAG-ATF6S1P− and pCI8abHA for 30 h (panels i–l), or transfected with 3XFLAG-ATF6S1P− and empty vector for 20 h, and then treated with (panels e–h) or without 5 μg/ml of tunicamycin (panels a–d) for an additional 4 h. Indirect immunofluorescence assay was performed as described. In panels e and i, white arrows point to the cells in which ATF6 exhibits Golgi localization, and gray arrows point to the cells in which ATF6 exhibits ER localization. In panel m, white arrowheads point to the Golgi, and gray arrowheads point to the site adjacent to the Golgi where 8ab co-localized with ATF6. (B) Co-immunoprecipitation of ATF6 and 8ab. Left panel, HeLa cells were transiently co-transfected with 8abHA and 3XFLAG-ATF6S1P− or empty vector. After 24 h, cell lysates were harvested and immunoprecipitated with anti-FLAG antibodies. 8abHA and ATF6 in the immunoprecipitates were detected using anti-HA or anti-FLAG antibodies as indicated. Right panel, HeLa cells were transiently co-transfected with HCV NS4B-HA and 3XFLAG-ATF6S1P− or empty vector. NS4B-HA or ATF6 contents in the immunoprecipitates were analyzed as left panel.